PATHOLOGY & ONCOLOGY RESEARCHVol. 6 No. 3, 2000

 Article

Morphometric Study of Tumor Angiogenesis as a New Prognostic Factor in Nasopharyngeal Carcinoma Patients

L RUBIO1, J S BURGOS2, C MORERA3, F J VERA-SEMPERE1

1Laboratory of Molecular Pathology, Service of Pathology II, University Hospital La Fe, Medical School of Valencia University, Valencia, Spain
2Center of Molecular Biology, ''Severo Ochoa'', CSIC-Autonomous University of Madrid, Madrid, Spain
3Service of ORL, University Hospital La Fe, Valencia, Spain

 

The aim of this study was to determine the possible prognostic significance of tumor angiogenesis (TA) in nasopharyngeal carcinoma (NPC) patients. Fifty-five NPC patients were evaluated in relation to survival. Endothelial cells were immunohistochemically stained with anti-von Willebrand factor (F-VIII), CD-31 and CD-34 antibodies, and microvessels counted in the most active areas of tumor neovascularization or hotspotsusing both a manual and an automatic method. Overall survival analysis calculated by the Kaplan–Meiertest revealed that both methods were correlated with a statistical significance between intratumoral microvessel density (IMD) and overall survival, using either manual (p=0.0141) or automatic counting (p=0.0117). Other angiogenic parameters studied were perimeter, roundness and accumulative area of the microvessels using a morphometric analyzer. Moreover, our results show that cases with high IMD demonstrated a prognostic significance in relation to the accumulative area (p=0.0072). Pathology & Oncology Research, Vol 6, Nr 3, 210-216, 2000

Key words: angiogenesis; morphometry; nasopharyngeal carcinona


Received: Mar 28, 2000; accepted: Jul 3, 2000
Correspondence: F J VERA-SEMPERE, Laboratory of Molecular Pathology, Service of Pathology II, University Hospital La Fe, Medical School of Valencia University, Avda. Campanar 21 Valencia 46009, Spain; Tel: 34-6-963862799, Fax: 34-6-963868789

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