PATHOLOGY & ONCOLOGY RESEARCHVol. 7 No. 2, 2001

 Article

DNA Ploidy Analyses in 218 Consecutive Pakistani Breast Cancer Patients: Does it Add Anything?

Enver KHAN1, Zohair MAPARA1, Shaista KHAN2, Naveeda ARSHAD1, Tariq SIDDIQUI3, Shahid PERVEZ1

1Department of Pathology, The Aga Khan University Medical Centre, Karachi, Pakistan
2Department of Surgery, The Aga Khan University Medical Centre, Karachi, Pakistan
3Department of Medicine, The Aga Khan University Medical Centre, Karachi, Pakistan

 

An analysis was made to evaluate the significance of DNA ploidy in the biology and prognosis of breast carcinoma. This was done by estimating the correlation of DNA ploidy with other established prognostic markers of breast cancer, namely tumor size, tumor grade, lymph node metastasis and S-phase fraction. From 1995 up to year 2000 ploidy analysis was performed on 218 consecutive cases of infiltrating breast carcinoma by flow cytometry using formalin fixed paraffin embedded material. From the laboratory record, data regarding other pathological variables was retrieved. No correlation could be found between DNA ploidy and tumor grade, nor could there be found a correlation with tumor size. For lymph node metastasis there was a significant difference between the proportion of aneuploids and diploids having metastasis in more than 4 lymph nodes. However, no significant difference was found in axillary lymph node positive and negative groups when number of positive lymph nodes was not taken into account. The mean value of S-phase fraction for the aneuploids and the diploids was also insignificantly different. In conclusion DNA ploidy alone did not add much to predict tumor behaviour in terms of known pathologic variables. Pathology & Oncology Research, Vol 7, Nr 2, 125-128, 2001

Key words: DNA ploidy; breast carcinoma; Pakistan


Received: Dec 6, 2000; accepted: May 28, 2001
Correspondence: Shahid PERVEZ, Department of Pathology, The Aga Khan University Medical Centre, Karachi , Pakistan; Tel: 9221-4930051/1554, Fax: 9221-4934294; E-mail: shahid.pervez@aku.edu

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