PATHOLOGY & ONCOLOGY RESEARCHVol. 7 No. 3, 2001

 Article

Immunohistochemical Assessment and Prognostic Value of Hepatitis B Virus X Protein in Chronic Hepatitis and Primary Hepatocellular Carcinomas using anti-HBxAgMonoclonal Antibody

József PÁL1, Csilla SOMOGYI1, Ágnes SZMOLENSZKY1, György SZEKERES2, József SÍPOS3, Géza HEGEDÜS4, Ilona MARTZINOVITS5, János MOLNÁR5, Péter NÉMETH1

1Department of Immunology and Biotechnology, University of Pécs, Faculty of Medicine, Pécs, Hungary
2Histopatology Ltd., Pécs, Hungary
3Department of Pathology, County Hospital of Zala,, Zalaegerszeg, Hungary
4Department of Pathology, County Hospital of Baranya, Pécs, Hungary
5Albert Szent-Györgyi Medical and Pharmaceutical Center, Department of Medical Microbiology, Faculty of Medicine, University of Szeged, Szeged, Hungary

 

Hepatitis B virus (HBV) is the most meaningful risk factor in chronic hepatitis, cirrhosis and primary hepatocellular carcinoma (PHC). The hepatitis B virus X protein (HBxAg) is a multifunctional protein with many important functions in hepatocellular carcinogenesis. A monoclonal anti-HBxAg antibody was developed in our laboratory and characterized by different methods. Using this antibody HBxAg was detected in formaldehyde fixed paraffin embedded tissue sections of 72 liver biopsies from patients with acute hepatitis, chronic hepatitis, cirrhosis and primary hepatocellular carcinoma. The co-expression of hepatitis B surface antigen (HBsAg), hepatitis B core antigen (HBcAg) and HBxAg was compared. The histological and cytological localization of the detected HBxAg showed a characteristic distribution in different stages of HBV infection. Strong and diffuse nuclear reaction was detected in PHC cases in contrast to the focal, cytoplasmic and nuclear labeling in the acute and chronic B hepatitis cases. Our antibody seems to be a suitable prognostic marker for routine pathohistological diagnosis and for comparative pathological and epidemiological research on the development of PHC. Pathology & Oncology Research, Vol 7, Nr 3, 178-184, 2001

Key words: primary hepatocellular carcinoma; hepatitis-B X protein; chronic B virus hepatitis; monoclonal anti-HBx antibody; immunohistochemistry


Received: Jul 11, 2001; accepted: Jul 25, 2001
Correspondence: Péter NÉMETH, Department of Immunology and Biotechnology, University of Pécs, Faculty of Medicine, Szigeti u. 12. Pécs H-7643, Hungary; E-mail: peter.nemeth@aok.pte.hu

Click here to get the full-text version in PDF!
ad