Her-2/neuGene Amplification Compared with HER-2/neu Protein Overexpression on Ultrasound Guided Core-Needle Biopsy Specimens of Breast Carcinoma

Handan KAYA1, Teresa RAGAZZINI2, Erkin ARIBAL3, Ilter GÜNEY4, Esin KOTILOGLU1

1Department of Pathology, Marmara University Hospital, Istanbul, Turkey
2Department of Oncology, University of Bologna, Bologna, Italy
3Department of Radiology, Marmara University Hospital, Istanbul, Turkey
4Department of Medical Biology, Marmara University Hospital, Istanbul, Turkey


Genomic amplification and oncoprotein overexpression of Her-2/neuwas studied on ultrasound core needle biopsy specimens of the infiltrative ductal carcinomas of the breast. We performed '' two colour '' fluorescence in situ hybridization (FISH) for Her-2/neuand chromosome 17 and compared the FISH results with the immunohistochemical overexpression of Her-2/neuprotein by 2 antibodies (DAKO HercepTest and the BioGenex monoclonal antibody AM 134-5M). Furthermore, following radical mastectomy with axillary dissection, Her-2/neustatus of the patients were compared with the well known histopathological prognostic factors such as histologic grade, tumor stage, lympho/ vascular invasion, surgical margin status and Paget’s disease. Amplification was demonstrated 27% of the cases. Her-2/neu protein overexpression was detected in 47% and 80% of the cases with CB11 and HercepTest respectively. We revealed statistically significant association between the tumor, oncoprotein expression and oncogene amplification (p<0.05). The results of our study showed that combination of IHC and FISH methods enhances the evaluation of tumor genetics at both gene and protein level for the analysis of Her-2/neuin breast carcinoma. Pathology & Oncology Research, Vol 7, Nr 4, 279-283, 2001

Key words: breast carcinoma; FISH; immunohistochemistry; Her-2/neu

Received: Nov 9, 2001; accepted: Nov 20, 2001
Correspondence: Handan KAYA, Department of Pathology, Marmara University Hospital, Altunizade Istanbul 81190, Turkey; Tel: 90 216 3271905, Fax: 90 216 3271905; E-mail:

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