PATHOLOGY & ONCOLOGY RESEARCHVol. 8 No. 2, 2002

 Article

Investigation of Microsatellite Instability in Turkish Breast Cancer Patients

Semra DEMOKAN1, Mahmut MUSLUMANOGLU2, H YAZICI1, Abdullah IGCI2, Nejat DALAY1

1Department of Basic Oncology, Oncology Institute, Capa, Istanbul, Turkey
2Department of Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey

 

Multiple somatic and inherited genetic changes that lead to loss of growth control may contribute to the development of breast cancer. Microsatellites are tandem repeats of simple sequences that occur abundantly and at random throughout most eucaryotic genomes. Microsatellite instability (MI), characterized by the presence of random contractions or expansions in the length of simple sequence repeats or microsatellites, is observed in a variety of tumors. The aim of this study was to compare tumor DNA fingerprints with constitutional DNA fingerprints to investigate changes specific to breast cancer and evaluate its correlation with clinical characteristics. Tumor and normal tissue samples of 38 patients with breast cancer were investigated by comparing PCR-amplified microsatellite sequences D2S443 and D21S1436. Microsatellite instability at D21S1436 and D2S443 was found in 5 (13%) and 7 (18%) patients, respectively. Two patients displayed instability at both marker loci. No association was found between MI and age, family history, lymph node involvement and other clinical parameters. Pathology & Oncology Research, Vol 8, Nr 2, 138-141, 2002

Key words: microsatellite instability; breast cancer


Received: Apr 12, 2002; accepted: Jun 6, 2002
Correspondence: Nejat DALAY, , ,

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