PATHOLOGY & ONCOLOGY RESEARCHVol. 5 No. 2, 1999

 Article

Elevated Hepatic Glucocorticoid Receptor Expression During Liver Regeneration in Rats

Csaba KARABÉLYOS1, Ottó DOBOZY1, Csaba SZALAI2, Katalin KLENJÁNSZKI1, Kornélia VARJÚ1, Ákos HADHÁZI1, Árpád KISS1, András Kristóf FÜLÖP1, Bálint MADARÁSZ1, András FALUS1

1Department of Genetics, Cell- and Immunobiology, Semmelweis University of Medicine, Budapest, Hungary
2Heim Pal, Children's Hospital, Budapest, Hungary

 

In rats within the first week of partial hepatectomy reconstruction of the normal histological structure of the liver already starts. To approach the possible role of endogenous glucocorticoids in the process of regeneration we measured the changes in the expression of steroid glucocorticoid receptor gene after various regeneration intervals. After partial hepatectomy, between 0.5–168 hours from the surgery, the gene expression (mRNA) of glucocorticoid receptor was determined by reverse transcription followed by PCR and normalized to that of glycerolphoshate dehydrogenase. Two peaks of glucocorticoid receptor mRNA were detected first, between 3 and 6 hours (first peak) and a second between 24 and 36 hours. Immunoreactive glucocorticoid receptor was detected by immunohistochemistry using monoclonal anti-glucocorticoid receptor. Three days after the surgery immunohistochemical studies showed substantially more immunoreactive GcR protein in the regenerated liver than in the controls. These semiquantitative data provide evidence suggesting elevation of glucocorticoid receptor expression during regeneration of liver at mRNA and protein levels. Pathology & Oncology Research, Vol 5, Nr 2, 107-109, 1999

Key words: glucocorticoid receptor; liver; regeneration; monoclonal antibody; mRNA


Received: Dec 15, 1998; accepted: Feb 27, 1999
Correspondence: András FALUS, Department of Genetics, Cell- and Immunobiology, Semmelweis University of Medicine, Nagyvárad tér 4. Budapest , Hungary; Tel: (36)-1-210-2929, Fax: (36)-1-303-6968; E-mail: faland@net.sote.hu

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