Paracrine Effects of IL-4 Transfection on TS/A Adenocarcinoma Cells Mediate Reduced In Vivo Growth

Sabrina PACOR1, Renato GAGLIARDI2, Paola SPESSOTTO2, Giuliano ZABUCCHI3, Gianni SAVA1

1Department of Biomedical Sciences, University of Trieste, Trieste, Italy
2Callerio Foundation, Institutes of Biological Research, Trieste, Italy
3Department of Physiology and Pathology, University of Trieste, Trieste, Italy


The in vitro/in vivo growth capacity and phenotype of TS/A and the IL4-transfected TS/A-IL4 cell lines were studied by cell cycle analysis, expression of ICAM-1/CD54, transferrin receptor/CD71 and E-cadherin and by histology of the primary tumors. TS/A-IL4, unlike the TS/A line, shows in vitro a marked increase in the fibroblastoid cell type and a decreased E-cadherin expression. Administration of conditioned medium containing IL4 obtained from the TS/A-IL4 cell line, stimulates CD54 expression in the TS/A cell line. TS/A-IL4 tumors grow more slowly in vivo and are ultimately rejected. These processes are accompanied by a marked increase in collagen and extracellular matrix proteins and increased recruitment and degranulation of mast cells. The paracrine effect of IL4, released by the transfected tumor cells, might be responsible for the reduced in vivo growth of the TS/A cell line in the presence of TS/A-IL4 cells. Pathology & Oncology Research, Vol 5, Nr 2, 110-116, 1999

Received: Jan 5, 1999; accepted: Mar 20, 1999
Correspondence: Gianni SAVA, Department of Biomedical Sciences, University of Trieste, via L. Giorgieri 7-9 Trieste 34127, Italy; Tel: +39-40-6763541, Fax: +39-40-577435; E-mail:

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