PATHOLOGY & ONCOLOGY RESEARCHVol. 5 No. 2, 1999

 Article

Analysis of p53 Mutation and Cyclin D1 Expression in Breast Tumors

János TANYI1, Kálmán TORY2, Agnes BÁNKFALVI3, Willibald SHRÖDER4, Werner RATH4, László FÜZESI2

1Ist Department of Gynaecology and Obstetrics, Semmelweis Medical University, Budapest, Hungary
2Institute of Pathology, Medical Faculty of the Technical University of Aachen, Aachen, Germany
3Department of Gynaecology, Medical Faculty of the Technical University of Aachen, Aachen, Germany
4Gerhard-Domagk-Institute of Pathology, Wilhems-University of Münster, Münster, Germany

 

P53 and cyclin D1 are interacting regulatory genes and both are frequently altered in breast cancer. We analysed p53 mutation by SSCP and sequencing methods as well as p53 protein accumulation immunohistochemically in 34 consecutively operated breast tumors. None of 4 fibroadenomas revealed p53 mutation or p53 protein accumulation. Mutation of p53 was present in 7 carcinomas. Immunohistochemistry revealed accumulation of p53 protein in 6 carcinomas and there was a significant correlation between p53 mutation and protein accumulation. Overexpression of cyclin D1 protein was observed in 11 carcinomas by immunohistochemistry and no correlation was observed between cyclin D1 overexpression and p53 mutation or accumulation. Our data support the concept that the p53-cyclin D1 signal pathway and the cyclin D1 cascade are disregulated in breast cancer. Pathology & Oncology Research, Vol 5, Nr 2, 90-94, 1999

Key words: breast cancer; fibroadenoma; p53; mutation; cyclin D1; immunohistochemistry


Received: Apr 2, 1999; accepted: May 10, 1999
Correspondence: János TANYI, Ist Department of Gynaecology and Obstetrics, Semmelweis Medical University, Baross str. 27. Budapest H-1088, Hungary; Tel: +36-1-216-0473, Fax: +36-1-117-6174; E-mail: tanyi©noi1.sote.hu

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