Genotypic Analysis in Primary Systemic Anaplastic Large cell Lymphoma

Árpád SZOMOR1, Prune ZENOU2, Daniel RODA2, Talal AL SAATI2, György CSANAKY3, László PAJOR4, Gábor KELÉNYI4, Georges DELSOL2, Hajna LOSONCZY1

1First Department of Medicine, University of Pécs, Pécs, Hungary
2Laboratoire d’Anatomie Pathologique, CNRS-UPR 2163, CHU Purpan, Toulouse, France
3Department of Pathology, Markusovszky Hospital, Szombathely, Hungary
4Institute of Pathology, University of Pécs, Pécs, Hungary


This report presents an experience of polymerase chain reaction (PCR) analysis of T-cell receptor g- and bgene (TCR g, TCR b), and immunoglobulin heavy chain (IgH) gene rearrangements in 9 cases of primary systemic anaplastic large cell lymphoma. We showed 2 clonal IgH, 2 TCR g, 1 TCR b rearrangements. The genotype was B/T-cell in 1, T-cell in 1, B-cell in 1 and null cell-type in 6 cases. We used reverse transcriptase PCR (RT-PCR) to detect t(2;5)(p23;q35) and t(1;2)(q25;p23) translocations. T(2;5) translocation was demonstrated in 2 cases, there was no t(1;2) translocation. In most cases the molecular genetic results were found to be in agreement with immunophenotypic data. Pathology & Oncology Research, Vol 9, Nr 2, 104-106, 2003

Key words: anaplastic large cell lymphoma; TCR g- and bgene rearrangement; IgH gene rearrangement; t(2;5)(p23;q35) and t(1;2)(q25;p23) translocation

Received: Feb 6, 2003; accepted: Mar 22, 2003
Correspondence: Árpád SZOMOR, First Department of Medicine, University of Pécs, Ifjúság u.13. Pécs 7624, Hungary, Fax: 36-72-536148; E-mail:

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