PATHOLOGY & ONCOLOGY RESEARCHVol. 10 No. 1, 2004

 Article

A WT1 Expressing Metastatic Human Kaposi Sarcoma Xenograft Model

Erzsébet RÁSÓ1, Lívia MÉSZÁROS2, Adriana ALBINI3, József TÍMÁR1

1Department of Tumor Progression, National Institute of Oncology, Budapest, Hungary
2Department of Diagnostic Pathology, National Institute of Oncology, Budapest, Hungary
3Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy

 

We have established a non-metastatic and a metastatic human Kaposi sarcoma (KS) xenograft model in SCID mice by injecting KS-Imm cells subcutaneously and intrasplenically, respectively. KS-Imm cells expressed endothelial markers, CD34 and vWF in vivo. Futhermore, we have shown that these cells express all the splice variants of the WT1 gene and WT1wt protein in vitro and in vivo detected by nested PCR and immunohistochemistry. WT1 expression in the peripheral blood was only detectable in case of metastatic KS model suggesting it as a molecular marker of progression. Pathology & Oncology Research, Vol 10, Nr 1, 22-25, 2004

Key words: Kaposi sarcoma; xenograft model; WT1 expression; metastasis


Received: Jan 6, 2004; accepted: Feb 20, 2004
Correspondence: József TÍMÁR, Department of Tumor Progression, National Institute of Oncology, Ráth György u. 7-9 Budapest H-1122, Hungary; Tel: 36-1-224-8786, Fax: 36-1-224-8706; E-mail: jtimar@oncol.hu

Click here to get the full-text version in PDF!
ad