PATHOLOGY & ONCOLOGY RESEARCHVol. 5 No. 3, 1999

 Article

Morphological-Histochemical Study of Intestinal Carcinoids and K-ras Mutation Analysis in Appendiceal Carcinoids

Helen PARASKEVAKOU, Angelica SAETTA, Kostas SKANDALIS, Sofia TSELENI, Aristides ATHANASSIADIS, Panayotis S DAVARIS

Department of Pathology, Medical School, University of Athens, Kifissia, Greece

 

Intestinal carcinoids are potentially malignant neoplasms. Their histogenesis and pathogenesis are currently uncertain. The morphological and histochemical characteristics of twenty intestinal carcinoids are studied. The primary sites of three mucin-producing tumors were examined by electron microscope. Furthermore 11 appendiceal carcinoids were analysed by the polymerase chain reaction (PCR) for the detection of ras and p53 point mutations. Microscopically all carcinoids were of mixed type. Focal mucin production was evident in three carcinoids that metastasised to regional lymph nodes. HID-Alcian blue staining proved that mucin in both primary and secondary foci did not belong to the sulphated group. The secretory granules and mucin droplets found in a single neoplastic cell suggest that carcinoids of the small intestine and some of the appendix arise from the endoderm. Neither ras nor p53 mutations were detected. It seems that ras oncogenes are probably not involved in the pathogenesis of appendiceal carcinoids. Pathology & Oncology Research, Vol 5, Nr 3, 205-210, 1999

Key words: carcinoids; morphology; histogenesis; p53 mutations; ras oncogenes


Received: Mar 25, 1999; accepted: May 18, 1999
Correspondence: Helen PARASKEVAKOU, Department of Pathology, Medical School, University of Athens, 29 Deliyianni Str. Kifissia GR-145 62, Greece; Tel: 00301-7771206, Fax: 00301-7781487; E-mail: asaettae@cc.uoa.gr

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