PATHOLOGY & ONCOLOGY RESEARCHVol. 4 No. 1, 1998

 Article

Expression of Invasion Markers CD44v6/v3, NM23 and MMP2 in Laryngeal and Hypopharyngeal Carcinoma

Gábor RÉPÁSSY1, Csaba FORSTER-HORVÁTH3, Attila JUHÁSZ4, Róza ÁDÁNY5, Anna TAMÁSSY2, József TÍMÁR2

1Department of Oto-Rhino-Laryngology, Semmelweis University of Medicine, Budapest, Hungary
21st Institute of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Budapest, Hungary
3Department of Molecular Pathology, Joint Research Organization of the Hungarian Academy of Sciences and the Semmelweis University of Medicine, Budapest, Hungary
4Department of Oto-Rhino-Laryngology, Debrecen Medical University, Debrecen, Hungary
5Department of Hygieny and Epidemiology, Debrecen Medical University, Debrecen, Hungary

 

Twelve laryngeal squamous cell carcinoma cases (7 laryngeal and 5 hypopharyngeal cancer; 15 samples) were analysed by immunohistochemistry for the expression of invasion markers CD44v6/v3, NM23 and matrix metalloproteinase, MMP2. The laryngeal epithelium showed CD44v6+/v3+/NM23-/MMP2- phenotype. When tumors were grouped into TNM categories the phenotype of the T2 and T3 tumors was similar, characterised by decreased CD44v3+ and lack of MMP2 expressions. Meanwhile the NM23 expression was more frequent in T3 tumors. In T4 stage the frequency of NM23 and MMP2 positive cases increased (5/6 and 4/6, respectively) but there was no correlation with the appearence of lymph node metastasis. Comparison of the phenotype of laryngeal and hypopharyngeal tumors, irrespective of the TNM stages, revealed characteristic differences: T2 stage laryngeal tumors showed decreased CD44v3 and occasional NM23 and MMP2 positivity, while in T3 stage these tumors were characterised by increased frequency of NM23 positivity. The phenotype of the hypopharyngeal tumors was significantly different with a high frequency of MMP2 positive cases (5/6) and NM23+/low CD44v3+ phenotype. The sharp differences in the phenotypes of laryngeal and hypopharyngeal carcinomas were connected to the differences in their invasive capacity unlike to the size of the tumors, since the T4 stage hypopharyngeal tumors had a significantly smaller size than laryngeal ones, even at lower stages. Pathology & Oncology Research, Vol 4, Nr 1, 14-21, 1998

Key words: laryngeal carcinoma; CD44v3; nm23; MMP2; immunohistochemistry


Received: Nov 15, 1997; accepted: Jan 10, 1998
Correspondence: József TÍMÁR, 1st Institute of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Üllõi út 26. Budapest H-1085, Hungary; Tel: (36)(1)1171070, Fax: (36)(1)1171074

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