PATHOLOGY & ONCOLOGY RESEARCHVol. 4 No. 2, 1998

 Article

Cytokine Sensitivity of Metastatic Human Melanoma Cell Lines - Simultaneous Inhibition of Proliferation and Enhancement of Gelatinase Activity

Andrea LADÁNYI1, Julianna O NAGY2, András JENEY2, József TÍMÁR2

1Department of Molecular Pathology, Joint Research Organization of the Hungarian Academy of Sciences and Semmelweis University of Medicine, Budapest, Hungary
21st Institute of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Budapest, Hungary

 

The effect of a panel of cytokines on the proliferation and type IV collagenase production was studied in four melanoma cell lines of different origin, tumorigenicity and metastatic capacity. TGF-?, TNF-? and to a lesser extent, IL-1? exhibited antiproliferative effect on the cell lines, with some lines showing varying degree of resistance. The sensitivity did not correlate directly with the origin or the biological behavior of the tumor lines, suggesting that cytokine resistance of advanced stage melanoma cells may be relative. IL-2, IL-10 and IL-12 displayed little or no effect on proliferation. The effect of cytokines on metalloproteinase production showed a cell line dependent pattern. Interestingly, those cytokines that exhibited the most pronounced antiproliferative activity, also proved most effective in stimulating collagenase secretion, often simultaneously, in the same line. The results indicate that pleiotropic cytokines can have positive and negative effects simultaneously on various steps of tumor progression. Pathology & Oncology Research, Vol 4, Nr 2, 108-114, 1998

Key words: melanoma; cytokines; proliferation; metalloproteinases


Received: Apr 8, 1998; accepted: May 12, 1998
Correspondence: Andrea LADÁNYI, Department of Molecular Pathology, Joint Research Organization of the Hungarian Academy of Sciences and Semmelweis University of Medicine, Üllõi út 26. Budapest H-1085, Hungary; Tel: (36)(1)2661638, Fax: (36)(1)3171074; E-mail: ladanyi@korb1.sote.hu

Click here to get the full-text version in PDF!
ad