PATHOLOGY & ONCOLOGY RESEARCHVol. 4 No. 3, 1998

 Article

Fibrinolytic Activity of Earthworms Extract (G-90) on Lysis of Fibrin Clots Originated from the Venous Blood of Patients with Malignant Tumors

Terezija M HRZENJAK1, Maja POPOVIC1, Ljerka TISKA-RUDMAN2

1Department of Biology, Faculty of Veterinary Medicine, Zagreb, Croatia
2Clinic for Tumors and Allied Diseases, Zagreb, Croatia

 

u-PA is secreted by the most malignant tumors. As a response to u-PA synthesis surrounding cells synthetize inhibitors of plasminogen activators for tissue protection. Plasminogen activators were found also in earthworm tissue. From the tissue homogenate of earthworm Eisenia foetida the glycolipoprotein mixture named G-90 was isolated. It contains two serine proteases (P I, P II) with fibrinolytic and anticoagulative activities. The fibrinolytic activity of G-90, P I and P II was tested in an in vitro euglobulinic test applied to fibrin clot from blood plasma of patients suffered from malignant tumors. G-90 and above-mentioned proteases applied in this study showed euglobulinic time proportionally with the concentrations of added substances. The influence of G-90 on the fibrinolysis rate does not depend only on its concentration, but depends too on histological type of tissue (organ) where the malignant tumors are located. Enzyme P I and P II do not show this activity. Pathology & Oncology Research, Vol 4, Nr 3, 206-211, 1998

Key words: fibrinolytic activity; earthworms extract; fibrin clot; malignant tumors


Received: Aug 10, 1998; accepted: Sep 8, 1998
Correspondence: Terezija M HRZENJAK, Department of Biology, Faculty of Veterinary Medicine, Heinzelova 55 Zagreb 10000, Croatia; Tel: 385-12390142, Fax: 385-1214697; E-mail: mthrzenj@rudjer.irb.hr

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