PATHOLOGY & ONCOLOGY RESEARCHVol. 4 No. 3, 1998

 Method

A New Method to Localize Acid Phosphatase Using the Confocal Laser-Scanning Microscope

Zsolt SZEGEDI1, Farhana AMIN2, Béla SZENDE1, Ivor Delme BOWEN2

1Department of Molecular Pathology, 1st Institute of Pathology and Experimental Cancer Research, Joint Research Organization of the Hungarian Academy of Sciences and Semmelweis University of Medicine, Budapest, Hungary
2Department of Pure and Applied Biology, University of Cardiff, Cardiff, UK

 

The aim of the study was to work out a technique for the detection of acid phosphatase enzyme activity by confocal laser-scanning microscope using the histochemical acid phosphatase detection method (after Barka and Anderson 1962, modified by Bowen and Lewis 1985) routinely used for light microscopy. The density and the distribution of enzyme reaction product is dependent on the incubation time, as shown by different confocal images or ELISA reader. The inhibition of the enzyme activity with metal ions shows the same profile known from the literature. This staining method seems to be useful to demonstrate subcellular distribution of the enzyme in the lysosomes and in the Golgi apparatus. Pathology & Oncology Research, Vol 4, Nr 3, 217-223, 1998

Key words: acid phosphatase activity; cytometry; cell image analysis; confocal laser-scanning microscope


Received: Jul 25, 1998; accepted: Sep 2, 1998
Correspondence: Zsolt SZEGEDI, Department of Molecular Pathology, 1st Institute of Pathology and Experimental Cancer Research, Joint Research Organization of the Hungarian Academy of Sciences and Semmelweis University of Medicine, Üllõi út 26. Budapest 1085, Hungary; Tel: +36-1-2661638/4440, Fax: +36-1-3171074; E-mail: szezso@korb1.sote.hu

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