PATHOLOGY & ONCOLOGY RESEARCHVol. 3 No. 1, 1997

 Article

Amount and Avidity of IgG Antibodies to Pseudomonas Aeruginosa Exotoxin A Antigen in Cystic Fibrosis Patients

Janja POLANEC1, Jan PATZER3, Jacek GRZYBOWSKI4, Milan STRUKELJ2, Zlatko P PAVELIC5

1Institute of Microbiology, Ljubljana, Slovenia
2Pediatric Clinic, Ljubljana, Slovenia
3Children's Memorial Hospital, Warsaw, Poland
4Military Institute of Hygiene and Epidemiology, Warsaw, Poland
5Department of Otolaryngology-Head and Neck Surgery, University of Cincinnati, Cincinnati, USA

 

Amount and avidity of serum IgG antibodies to Pseudomonas aeruginosa exotoxin A in sera of 31 patients with cystic fibrosis (CF) was studied. Eight patients had P. aeruginosa isolated from the sputum on multiple occasions, while from 23 patients no P. aeruginosa was isolated. Amount of IgG antibodies to P. aeruginosa exotoxin A were significantly increased in the serum of patients with P. aeruginosa pulmonary colonization (p<0.0001). On the contrary, serum IgG avidity in the colonized and in the non-colonized CF patients was low (<10) and was statistically different when compared to the 30 age-matched healthy controls (p<0.0001). There was no change in IgG avidity in six chronically infected CF patients from whom we obtained serum samples after half a year period (p=0,55). Pathology & Oncology Research, Vol 3, Nr 1, 26-29, 1997

Key words: ELISA; avidity; cystic fibrosis; exotoxin A; Pseudomonas aeruginosa


Received: Nov 25, 1996; accepted: Feb 13, 1997
Correspondence: Zlatko P PAVELIC, Department of Otolaryngology-Head and Neck Surgery, University of Cincinnati, Cincinnati 45267-0528, USA; Tel: (513)5586275, Fax: (513)5585203

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