Immunohistochemical and Ultrastructural Analysis of a Mammary Cystic Hypersecretory Carcinoma

Gábor CSERNI1, Szabolcs VIRÁGH2

1Department of Pathology, Bács-Kiskun County Hospital, Kecskemét, Hungary
2Institute of Pathology, Haynal Imre University of Health Sciences, Budapest, Hungary


Cystic hypersecretory carcinoma (CHC) is a rare variant of intraductal carcinoma. A CHC in a 50-year-old woman was excised and processed for light and electron microscopy and immunohistochemistry. The tumor had a marked cystic appearance. The walls of the cysts consisted of epithelial and myoepithelial cells and a well-developed basement membrane. The epithelial cells contained well-developed rough-surfaced endoplasmatic reticulum and Golgi apparatus. Secretory granules were not detected, with the exception of a few mucus-producing cells. The secretion was predominantly homogenous, reminiscent of thyroid colloid, and demonstrated distinct PAS positivity. The cells displayed a strong labeling with epithelial membrane antigen (EMA) and EMA-positive structures were observed within the intraluminal secretion, too. Some of these were stained by alcian blue. In addition, the colloid-like material was admixed with mucus showing a filamentous internal structure and lipid droplets resulting in some heterogenity of the secretion. Intraductal micropapillary proliferation in some of the cysts and adjacent nondistended ducts was a further defining feature of the tumor. Steroid hormone receptor and Ki-67 proliferation marker immuno his Tochemistry showed scattered positivity among the tumor cells. These results are in agreement with previous observations and further clarify the nature of this low-grade in situ cancer. Pathology & Oncology Research, Vol 3, Nr 4, 287-292, 1997

Key words: breast; cystic hypersecretory carcinoma; electronmicroscopy; immunohistochemistry

Received: Nov 27, 1997; accepted: Dec 4, 1997
Correspondence: Gábor CSERNI, Department of Pathology, Bács-Kiskun County Hospital, Nyíri út 38. Kecskemét H-6000, Hungary; Tel: (36)(76)481781, Fax: (36)(76)481219; E-mail:

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