Lack of Correlation Between Survival and Allele Loss on Chromosome 7q31-32 in Primary Breast Cancer

Marianna SZTAN1, István BESZNYÁK2, Tibor KOVÁCS2, József TÓTH3, Irén SZAMEL4, Edit OLÁH1

1Department of Molecular Biology, National Institute of Oncology, Budapest, Hungary
2Department of Surgery, National Institute of Oncology, Budapest, Hungary
3Department of Pathology, National Institute of Oncology, Budapest, Hungary
4Department of Clinical Research, National Institute of Oncology, Budapest, Hungary


High incidence of loss of heterozygosity (LOH), affecting the 7q31-32 chromosome region in sporadic primary human breast carcinomas suggests the presence of a tumor suppressor gene in this region which seems relevant to the development of breast cancer. To further determine the possible role of this region in the pathogenesis of human primary breast cancer and association with survival, LOH analysis was performed on 52 primary breast cancer patients using a set of highly polymorphic microsatellite markers. Our panel contained twenty biopsy cases of unknown survival, nineteen cases with more than five years survival and fourteen cases with less than two years survival. Corresponding normal and tumor DNAs were analyzed by polymerase chain reaction (PCR). The data presented here demonstrate that all patients were informative at least at one locus and 20 (38%) out of 53 cases showed LOH at one or more loci on chromosome 7q31-32. Relatively high incidence of LOH (34%) was detected at the D7S522 microsatellite marker located near to the cMet proto-oncogene while lower frequencies were observed at D7S523 (19%) and D7S495 (17%) loci, supporting the existence of a putative tumor suppressor gene at the chromosome 7q31.1 region. Our results suggest that allelic imbalance on 7q may occur at an early stage of breast carcinogenesis, as no correlation was observed between allelic loss and clinico-pathological data. Pathology & Oncology Research, Vol 2, Nr 1, 48-51, 1996

Key words: chromosome 7q; tumor suppressor gene; loss of heterozygosity; primary breast carcinoma

Received: Feb 9, 1996; accepted: Feb 27, 1996
Correspondence: Edit OLÁH, Department of Molecular Biology, National Institute of Oncology, Ráth György u. 7. Budapest H-1525, Hungary; Tel: 36-1-1562402, Fax: 36-1-1550125

Click here to get the full-text version in PDF!