PATHOLOGY & ONCOLOGY RESEARCHVol. 2 No. 2, 1996

 Article

Effect of Ribonuclease A and Deoxyribonuclease I on Immunostaining of Ki-67 in Cultured Melanoma Cells

Jamal BENFARES1, Agnés LE TOURNEAU2, Josée AUDOUIN2, György SZEKERES3

1R&D Laboratory, Antibody Department, Immunotech, Marseille, France
2Laboratory of Pathology, Hótel-Dieu, Paris, France
3Laboratory of Histopathology, Pécs, Hungary

 

Immunostaining of the cell cycle-associated Ki-67 antigen was studied, using the Ki-67-specific MIB-1 monoclonal antibody on slides prepared by cytocentrifugation of cultured A375 melanoma cells. Immunomorphological analysis of the Ki-67 immunostaining pattern of both nuclear and nucleolar locations was carried out following pre-treatment of the slides including ribonuclease and deoxyribonuclease pre-digestion of the cells. Immunostaining of nucleolar Ki-67 was reduced by ribonuclease pre-digestion, but was not altered by deoxyribonuclease pre-treatment. Ribonuclease did not reduce the staining intensity of Ki-67 in the nuclear matrix, but the intensity decreased after deoxyribonuclease pre-digestion. We suggest that the Ki-67 molecule may play an important role in ensuring contact between nuclear DNA and nucleolar RNA during transcriptional processes in cell proliferation. Pathology & Oncology Research, Vol 2, Nr 2, 63-65, 1996

Key words: Ki-67; immunocytochemistry; enzymatic pre-digestion; proliferating cells


Received: Mar 21, 1996; accepted: Apr 24, 1996
Correspondence: György SZEKERES, Laboratory of Histopathology, , Akác u. 8. Pécs H-7632, Hungary; Tel: 36-72-256493, Fax: 36-72-256493

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