PATHOLOGY & ONCOLOGY RESEARCHVol. 2 No. 4, 1996

 Report

Determination of HIV-1 Subtypes in Hungary by Synthetic Peptides Representing the V3 Loop of env

Károly NAGY, Éva BARABÁS, Viktória VÁRKONYI, Attila HORVÁTH

Department of AIDS and Human Retroviruses, National Institute of Dermato-Venereology, Budapest, Hungary

 

For the determination of HIV-1 diversity and serotyping of HIV-1 subtypes, an enzyme immunoassay was developed based on synthetic peptides representing immunodominant epitopes of the V3 loop of HIV-1 subtypes A, B, C and E, respectively. Sera from 53 asymptomatic HIV-1 infected individuals were tested for their pattern of binding reactivity to the synthetic peptides. 45/52 (85%) of the sera reacted exclusively to V3 peptide representing HIV-1 B subtypes, 4/52 (7.6%) of the sera showed cross reacticity to A/B peptides and 1/52 (1.9%) of the sera reacted with both A and C peptides. No single reactivity with subtype A or E peptides have been observed. Results together with nucleotide sequence analysis of the V3 region of clinical isolates suggest that HIV-1 infection in Hungary has been induced predominantly by strains belonging to HIV-1 subtype B. Pathology & Oncology Research, Vol 2, Nr 4, 268-271, 1996

Key words: HIV-1; subtypes; Hungary


Received: Sep 1, 1996; accepted: Sep 20, 1996
Correspondence: Károly NAGY, Department of AIDS and Human Retroviruses, National Institute of Dermato-Venereology, Mária u. 41. Budapest H-1085, Hungary; Tel: 36-1-2660465, Fax: 36-1-2104874

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