PATHOLOGY & ONCOLOGY RESEARCHVol. 10 No. 2, 2004

 Article

The Pattern of Cytokine Gene Expression in Human Colorectal Carcinoma

Anna CSISZÁR1, Tamás SZENTES2, Bea HARASZTI3, Annamária BALÁZS1, Gyõzõ G PETRÁNYI3, Éva PÓCSIK3

1Department of Physiology, New York Medical College, Valhalla, USA
2Department of Surgery, Szent Imre Hospital, Budapest, Hungary
3Laboratory of Tumor Immunology, National Medical Center, Budapest, Hungary

 

Systemic and local cytokine environment may modulate the immunogenicity of colorectal cancer cells, and affect anti-tumor immune functions of tumorinfiltrating lymphocytes. We therefore investigated cytokine mRNA expression patterns in tumors and peripheral blood mononuclear cells (PBMC) from patients with colorectal adenocarcinoma. IL-2, IFN-g, tumor necrosis factor-a (TNF-a), IL-4, IL-6, IL-8, IL-10 and IL-1b mRNAs in single cell suspension of freshly isolated colorectal cancer tissue were studied by RT-PCR. Frequencies of cytokine gene expression were compared to those in normal colonic mucosa from tumor patients. The frequencies of IL-2, IFN-g, IL-4 and IL-10 gene expression were also determined in peripheral blood mononuclear cells from patients with colorectal adenocarcinoma and compared to those of healthy individuals. Tumor samples were more frequently positive for IFN-g, IL-2, TNF-a and IL-10 gene expression than normal mucosa (p=0.0001, p=0.0118, p=0.001 and p<0.0001, respectively). Frequencies of IL-2 and TNF-a gene expressions were significantly higher in tumors with a diameter <5 cm, than in those with a diameter >5 cm. The genes for IL-6, IL-1b and IL-8 were commonly expressed in both tumor tissue and normal colonic mucosa. IFN-g transcripts were detected in more PBMC samples from patients with colorectal cancer than those from normal controls (p=0.0449). Thus, colorectal cancer tissue is characterized by a specific pattern of cytokine gene expression. It is likely that multiple interactions between pro- and anti-inflammatory cytokines regulate tumor growth and the functional activity of tumor-infiltrating lymphocytes. Pathology & Oncology Research, Vol 10, Nr 2, 109-116, 2004

Key words: cytokine; gene expression; colorectal carcinoma; RT-PCR


Received: Mar 12, 2004; accepted: May 12, 2004
Correspondence: Éva PÓCSIK, Laboratory of Tumor Immunology, National Medical Center, 64 Diószegi út Budapest H-1113, Hungary; Tel: +36-1-372-4361, Fax: +36-1-466-7020; E-mail: pocsik@ohvi.hu

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