PATHOLOGY & ONCOLOGY RESEARCHVol. 10 No. 3, 2004

 Article

Apoptosis in Transitional Cell Carcinoma of Bladder and its Relation to Proliferation and Expression of P53 and Bcl-2

Zahra AMIRGHOFRAN2, Ahmad MONABATI3, Abdolaziz KHEZRI4, Zahra MALEK-HOSSEINI1

1Departments of Immunology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
2Medicinal and Natural Product Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Pathology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
4Department of Urology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran

 

Transitional cell carcinoma of bladder (TCC) is a relatively common cancer among men. Tumor progression is associated with expression or modulation of several gene products that control apoptosis and proliferation. Apoptosis is a negative growth regulatory mechanism in tumors. The aim of this study is to examine apoptosis and related regulatory molecular markers in a group of patients with TCC. Paraffinembedded tissues from 49 patients with TCC were examined for the expression of bcl-2, p53 and Ki-67 by immunohistochemistry. Apoptosis was detected by TUNEL method. Correlation between apoptotic index (AI), proliferation index (PI) and bcl-2 and p53 expression with each other and with pathological grade was determined. Apoptosis was observed in 28.1% of TCC cases. The mean AI of all cases was 13.7±24. No correlation was found between apoptosis and differentiation status of carcinoma. Bcl-2 expression was weakly detected in only one sample. P53 expression was detected in 26 of cases with mean staining index of 102±96. A significant correlation between p53 and Ki-67 staining indices was observed (r=0.521, p=0.001). Both p53 and Ki-67 expression showed a good association with the pathological grade (p=0.0001 and p=0.004, respectively). None of the markers showed significant correlation with AI and no correlation was found between the ratio of AI to PI and other parameters either. In conclusion, the frequency of apoptosis in TCC of bladder appears not to be associated with tumor grade, and with bcl-2, p53 and Ki-67 expression. Pathology & Oncology Research, Vol 10, Nr 3, 154-159, 2004

Key words: Transitional cell carcinoma of bladder; apoptosis; bcl-2; p53


Received: Mar 31, 2004; accepted: Aug 12, 2004
Correspondence: Zahra AMIRGHOFRAN, Departments of Immunology, Medical School, Shiraz University of Medical Sciences, Shiraz , Iran; Tel: 98-711-2304069, Fax: 98-711-2359847; E-mail: amirghz@sums.ac.ir

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