PATHOLOGY & ONCOLOGY RESEARCHVol. 10 No. 3, 2004

 Method

Ideal Cooling Process for Paraffin-Embedded Tissues

Ferenc BRITTIG1, Philip WEINKAUF2, Hannelore KÄRCHNER3

1Pathology Laboratory, County Hospital, Veszprém, Hungary
2Design engineer,
3Design engineer,

 

Back in the seventies everybody was convinced that it was no longer necessary to cool paraffinembedded tissues, because of the new advances in the production of paraffin. The reason for this assumption was the addition of plastic polymers and dimethyl sulfoxide. The quality of tissue sectioning improved because of these additives. The daily routine in the histology laboratories shows that it is impossible to produce good quality cutting without cooling. Cooling the paraffin by means of cooling plates or ice dishes creates drastic improvement; the cutting quality improves and it is much easier. Both improvements speed up the process and save time for the technicians and the physician. Long-term study indicates that not only the temperature but also the methodology of cooling and the cooling rate are playing a decisive role in the cutting quality. Pathology & Oncology Research, Vol 10, Nr 3, 172-173, 2004

Key words: sectioning; paraffin block; cooling technique


Received: Jul 1, 2004; accepted: Aug 22, 2004
Correspondence: Ferenc BRITTIG, Pathology Laboratory, County Hospital, Veszprém H-8200, Hungary; Tel: (36) 88-556-000, Fax: (36) 88-556-209

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