PATHOLOGY & ONCOLOGY RESEARCHVol. 10 No. 4, 2004

 Article

Prognostic Significance of Dysadherin Expression in Cervical Squamous Cell Carcinoma

Dan WU1, Yuhuan QIAO2, Gunnar B KRISTENSEN4, Shanshan LI3, Gunhild TROEN1, Ruth HOLM1, Jahn M NESLAND1, Zhenhe SUO1

1Department of Pathology, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway
2Department of Obstetrics and Gynecology, The First Teaching Hospital, Zhengzhou University, Henan, China
3Department of Pathology, The First Teaching Hospital, Zhengzhou University, Henan, China
4Department of Gynecologic Oncology, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway

 

The protein and mRNA expression of dysadherin was studied in a series of squamous cell cervical carcinomas, and their clinicopathological associations and prognostic value were explored. Immunohistochemistry was used to assess protein expression of dysadherin in 206 patients with squamous cell cervical carcinoma, FIGO stage Ia-IVb. Frozen tissues from 20 cases in which the tumors showed variable dysadherin protein expression were used for laser capture microdissection (LCM) and processed for RTPCR detection of dysadherin mRNA. Immunohistochemically, all the dysadherin-positive staining was membranous. Positive cell membranous dysadherinpositive staining was often observed at the edge of tumor nests, although strong immunoreactivity throughout whole tumor nests was also seen in some tumors. Basal cells of the normal cervical epithelia were positive for dysadherin while its expression in the squamous cell cervical carcinomas was variable. Among the 206 tumors, 23 (11.2%) were negative, 53 (25.7%) were scored 1+, 54 (26.2%) were scored 2+ and 76 (36.9%) were scored 3+. In the 20 tumors analyzed, mRNA expression of dysadherin basically corresponded to its protein expression. No significant correlation between expression of dysadherin and age, FIGO stage or lymph node status was observed. Higher level of dysadherin expression, however, was significantly associated with shorter overall survival (p=0.04). We conclude that there is dysadherin protein expression in basal and parabasal cells of normal cervical epithelia, and higher level of dysadherin protein expression in squamous cell cervical carcinoma is predictive of a shorter overall survival, indicating that dysadherin may be a valuable prognostic marker in cervical carcinoma. Pathology & Oncology Research, Vol 10, Nr 4, 212-218, 2004

Key words: dysadherin; squamous cell cervical carcinoma; immunohistochemistry; RT-PCR; laser capture microdissection


Received: Sep 15, 2004; accepted: Nov 23, 2004
Correspondence: Zhenhe SUO, Department of Pathology, The Norwegian Radium Hospital, University of Oslo, Montebello Oslo 0310, Norway; Tel: +00-47-22935588, Fax: +00-47-22730164; E-mail: zhenhe.suo@labmed.uio.no

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