PATHOLOGY & ONCOLOGY RESEARCHVol. 10 No. 4, 2004

 Article

Establishment and Characterization of a Human Large Cell Lung Cancer Cell Line with Neuroendocrine Differentiation

Sedigheh SHARIFZADEH1, S Mohammad OWJI2, Abdul Mohammad PEZESHKI4, Zahra MALEK-HOSEINI1, Perikala Vijayananda KUMAR2, Sied Mohammad Ali GHAYUMI3, Abbas GHADERI4

1Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran

 

Characterization of a human lung cancer cell line is reported. This cell line was established from a patient referred to Nemazi Hospital of Shiraz University of Medical Sciences with a diagnosis of poorly differentiated carcinoma. Sterile sample from peritoneal effusion was taken and immediately cultured in RPMI-1640 medium containing 20% FBS, at 37C with 5% CO2. This cell line has been in continuous culture for more than one year and has been named as Mehr-80. Several features of the cell line were investigated, including growth characteristics, electron microscopic features, cloning efficiency in soft agar, expression of various antigenic markers, chromosomal and DNA analysis. On the basis of morphological and immunohistochemical analysis of Mehr-80, it is possible to conclude that this cell line is characterized by features similar to those reported for large cell carcinoma with neuroendocrine differentiation (LCCND). This cell line will be a valuable in vitro tool for further studies on lung cancers. Pathology & Oncology Research, Vol 10, Nr 4, 225-230, 2004

Key words: cell line; lung cancer; large cell carcinoma; neuroendocrine differentiation


Received: Feb 5, 2004; accepted: Nov 18, 2004
Correspondence: , Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz , Iran; Tel: +98-711-2303687, Fax: +98-711-2304952; E-mail: ghaderia@sums.ac.ir

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