Polymorphisms of the XRCC1 DNA Repair Gene in Head and Neck Cancer

Semra DEMOKAN1, Deniz DEMIR2, Yusufhan SUOGLU2, Erkan KIYAK2, Ugur AKAR3, Nejat DALAY1

1Oncology Institute, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
2Department of Otolaryngology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
3Department of Medical Biology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey


Inherited polymorphisms in the genes controlling the cell cycle or functioning in the DNA repair mechanisms may impair their function and contribute to genetic susceptibility. Abnormalities in the DNA repair have been reported in head and neck cancer. The XRCC1 gene functions in singlestrand break and base excision repair processes. In this study, two polymorphisms of the XRCC1 gene, Arg194Trp and Arg399Gln were investigated in 95 patients with head and neck carcinoma. The polymorphic regions were amplified by PCR followed by digestion with methylation-specific restriction enzymes, and analyzed electrophoretically. Genotype and allele frequencies were calculated, and association with cancer risk or clinical parameters was investigated. No association was observed between the genotypes and head and neck cancer for either polymorphism. Distribution of the alleles did not significantly differ between the patients and the control group. A significant association was only found for the Trp194 allele among the smoking individuals. Our data indicate that the Arg194Trp and Arg399Gln polymorphisms do not confer a significant risk for head and neck carcinogenesis. Pathology & Oncology Research, Vol 11, Nr 1, 22-25, 2005

Key words: polymorphism; XRCC1; head and neck cancer

Received: Nov 24, 2004; accepted: Jan 5, 2005
Correspondence: Nejat DALAY, Oncology Institute, Istanbul Medical Faculty, Istanbul University, Capa Istanbul 34390, Turkey; Tel: 90 212 5313100, Fax: 90 216 3387809; E-mail:

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