Detection of TNFa Expression in the Bone Marrow and Determinationof TNFa Production of Peripheral Blood Mononuclear Cells in Myelodysplastic Syndrome

Lenke MOLNÁR1, Tímea BERKI2, Alizadeh HUSSAIN1, Péter NÉMETH2, Hajna LOSONCZY1

11st Department of Internal Medicine, University Medical School of Pécs, Pécs, Hungary
2Department of Immunology and Biotechnology, University Medical School of Pécs, Pécs, Hungary


TNFa is a highly active cytokine which plays an important role in the regulation of apoptotic cell death, a mechanism involved in the pathophysiology of myelodysplastic syndrome (MDS). In this study we investigated the expression of TNFa of the bone marrow trephine biopsies by immunohistochemical method and the TNFa production of peripheral blood mononuclear cells by ELISA method in 15 patients affected by MDS. Five of seven patients without excess of blasts showed high or intermediate TNFa expression in the bone marrow biopsies, whereas two patients with excess of blasts were negative and one had low expression. The five CMML patients revealed low or intermediate expression. The production of TNFa by the PBMC was analysed in 10 patients, four patients with RA and two with CMML produced higher level of TNFa which increased after stimulation with phorbol myristic acetate, but none of the RAEB patients revealed increase in TNFa production. In conclusion we suppose that increased TNFa expression and production by PBMC may be an indirect evidence of the role of increased apoptosis in low risk MDS patients. Pathology & Oncology Research, Vol 6, Nr 1, 18-23, 2000

Key words: Myelodysplastic syndrome; MDS; apoptosis; TNFa

Received: Dec 22, 1999; accepted: Jan 15, 2000
Correspondence: Lenke MOLNÁR, 1st Department of Internal Medicine, University Medical School of Pécs, Ifjúság str. 13 Pécs 7624, Hungary

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