High Production of SPARC/osteonectin/BM-40 in Mouse Metastatic B16 Melanoma Cell Lines

Yasumasa KATO4, Francis FRANKENNE1, Agnés NOËL1, Naoki SAKAI2, Yoji NAGASHIMA3, Shinri KOSHIKA4, Kaoru MIYAZAKI5, Jean-Michel FOIDART1

1Laboratory of Tumor and Developmental Biology, Faculty of Medicine, University of Liege, Liege, Belgium
2Department of Urology, Yokohama City University School of Medicine, Yokohama, Japan
3Department of Pathology, Yokohama City University School of Medicine, Yokohama, Japan
4Department of Biochemistry, Kanagawa Dental College, Yokosuka, Japan
5Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan


Production of SPARC/osteonectin/BM-40 was determined in mouse B16 melanoma clones BL6 and F10 (high metastatic) and F1 (low metastatic). SPARC was produced greater amount in BL6 and F10 than in F1 cells, showing a good agreement with their metastatic potentials. Moreover, SPARC production was not influenced by culture pH, even in the acidic conditions (=pH 5.9). Although tumor tissues show often low pH due to excessive amount of acidic metabolites such as lactate, most studies have been done in neutral pH. High SPARC production in the acidic medium, therefore, is thought to be an important potential for tumor invasive behaviour. Pathology & Oncology Research, Vol 6, Nr 1, 24-26, 2000

Key words: SPARC; osteonectin; BM-40; melanoma; metastasis

Received: Jul 30, 1999; accepted: Jan 10, 2000
Correspondence: Yasumasa KATO, Department of Biochemistry, Kanagawa Dental College, 82 Inaoka-cho Yokosuka 238-8580, Japan; Tel: +81-(0)468-22-8840, Fax: +81-(0)468-22-8839; E-mail:

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