PATHOLOGY & ONCOLOGY RESEARCHVol. 6 No. 1, 2000

 Article

Unique Morphological Alterations of the HTLV-I Transformed C8166 Cells by Infection with HIV-1

Joseph ONGRÁDY1, Helen M LAIRD2, Joseph F SZILÁGYI3, Attila HORVÁTH1, Mauro BENDINELLI4

1National Institute of Dermato-Venerology, Budapest, Hungary
2Department of Veterinary Pathology, University of Glasgow, Glasgow, UK
3Institute of Virology, University of Glasgow, Glasgow, UK
4Department of Biomedicine, University of Pisa, Pisa, Italy

 

C8166 cells express T lymphocyte markers, a monocyte-specific esterase, taxpolypeptide of HTLV-I. In spite of this transactivator, their HIV-1 yield is low. Their culture conditions were modified, and infected cells were immobilized on a poly-L-lysine sheet under semisolid overlays to study their phenotypic alterations and HIV-1 production by microscopy and electron microscopy. Another lymphoid cultures (MT-4, CEM, CEM-ss, AdCEM) similarly treated were infected with either HIV-1/RF or IIIB. Specificity of HIV-1 was compared to the effects of vesicular stomatitis virus (VSV). Unlike other cultures, HIV-1/RF infected C8166 cells in Eagle’s MEM exhibited surface projections resembling hairy leukemia cells, which was followed by balloon degeneration and apoptosis. Immobilized HIV-1 infected cultures formed flat syncytia with several interdigitating dendritic projections. Syncytia shrunk with condensed nuclear material and axon-like filaments characteristic for infected macrophages. VSV induced enlargement and necrotic lysis of all cell types. Early postinfection with HIV-1, electron microscopy revealed irreversible membrane fusion above cell nuclei, and transient fusion between filaments. Transient presence of coated vesicles containing intact HIV-1 particles, Birbeck granule-like structures of Langerhans cells, fibrillar-lamellar structures resembling hairy leukemia or Sézary cells were detected. Late postinfection, high proportion of HIV-1 bud from polarized cytoplasm was empty particle, while that bud and entrapped in cytoplasmic vacuoles contained two or multiple cores in a fused envelope. The effect of early gene products of HIV-1 on HTLV-I and C8166 cells might elicit their latent potentials for monocyte or interdigitating dendritic cells, while in the later phase HTLV-I products might alter HIV-1 virion assembly. Pathology & Oncology Research, Vol 6, Nr 1, 27-37, 2000

Key words: C8166; HIV-1; leukemia; surface projections; cellular mimicry; aberrant virions


Received: Jun 10, 1999; accepted: Jan 10, 2000
Correspondence: Joseph ONGRÁDY, National Institute of Dermato-Venerology, Mária utca 41. Budapest 1085, Hungary; Tel: +361-266-0465 / 5717, Fax: +361-267-6974; E-mail: ongjos@hotmail.com

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