Microvessel Count, Proliferating Cell Nuclear Antigen and Ki-67 Indices in Gastric Adenocarcinoma

Gülsüm Özlem ELPEK1, Tekinalp GELEN1, Nazif Hikmet AKSOY1, Tuncer KARPUZOGLU2, Nuran KELES1

1Department of Pathology, Akdeniz University, Medical School, Antalya, Turkey
2Department of General Surgery, Akdeniz University, Antalya, Turkey


The aim of the present study was to immunohistochemically investigate the prognostic value of neovascularization (expressed as microvessel count-MVC) and tumor cell proliferation (expressed as PCNA labeling index – PLI and Ki-67 labeling index – KLI) in gastric adenocarcinoma. Correlations with clinicopathologic features were also evaluated. Tumor specimens from 74 patients diagnosed as gastric adenocarcinoma were included in this study. Formalin fixed, paraffin embedded tissue sections stained immunohistochemically with F-VIII, PC10 and MIB-1 monoclonal antibodies. By ocular grid subdivided into 100 areas, number of microvessels and PC10, MIB-1 positive and negative cells were counted at x400 magnification. Chi-square test, Kaplan-Meier method and cox regression analysis were used for statistical analysis. The results showed that, MVC and PLI had a significant correlation with invasion and lymph node metastasis. The prognosis was significantly worse in patients with high MVC (>14 ) and with high PLI (>49%). However any relationship was not observed between KLI (38%) and clinicopathologic parameters, so KLI failed to predict the prognosis. Cox model showed that, MVC and PLI were independent prognostic variables. Ki-67 labeling index in gastric carcinomas has no prognostic relevance. However, the evaluation of microvessel count and proliferating cell nuclear antigen index in gastric carcinomas could be reliable indicators of prognosis. Pathology & Oncology Research, Vol 6, Nr 1, 59-64, 2000

Key words: microvessel count; angiogenesis; proliferation; gastric carcinoma

Received: Nov 30, 1999; accepted: Jan 20, 2000
Correspondence: Gülsüm Özlem ELPEK, Department of Pathology, Akdeniz University, Medical School, Týp Fakültesi, Patoloji ABD, Yeni Týp, Dekanlýk Antalya 07070, Turkey; Tel: +90 242 227 44 88, Fax: + 90 242 227 44 82; E-mail:

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