PATHOLOGY & ONCOLOGY RESEARCHVol. 6 No. 2, 2000

 Article

Moc-31, Fibronectin and CEA in the Differential Diagnosis of Malignant Effusions: An Immunocytochemical Study

Pauline ATHANASSIADOU1, Maria GONIDI1, Anna LIOSSI1, Efthalia PETRAKAKOU1, Lydia NAKOPOULOU1, Cherry ZERVA1, Peter ATHANASSIADES2

1Pathology Laboratory, Cytology Department, University of Athens, Athens, Greece
2Clinical Therapeutics, Alexandra Hospital, University of Athens, Athens, Greece

 

In discriminating benign and malignant origins of cytologically suspicious effusion smears a panel of antibodies against carcinoembryonic antigen (CEA), Fibronectin (F) and MOC-31 was used with immuno-cytochemical techniques. One hundred and thirty seven effusions were studied of which 107 had a malignant and 30 a benign aetiology as determined by clinical and histological examination. Cytologically 24 were diagnosed as benign, 97 as malignant and 14 as suspicious. Staining for F was positive in all effusions of benign and 3 of malignant origin. MOC-31 was positive in 95 (88.8%) of effusions of malignant origin but none of benign origin. Positive CEA was observed in 43% of effusions of malignant origin and in 10 of benign origin. The combination of MOC-31 positivity measured the sensitivity and specificity of the cytological examination in cases where the cytological examination result was suspicious as did F positivity improve the sensitivity for a benign origin of the effusion. Positivity or negativity for CEA is less valuable than the other parameters. Pathology & Oncology Research, Vol 6, Nr 2, 100-103, 2000

Key words: MOC-31; CEA; fibronectin; immunocytochemistry; effusions; reactive atypical mesothelial cells


Received: Oct 5, 1999; accepted: Mar 5, 2000
Correspondence: Pauline ATHANASSIADOU, Pathology Laboratory, Cytology Department, University of Athens, 75 M.Asias Str. Athens GR-115 27, Greece; Tel: +30-1-7781663, Fax: +30-1-7781487

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