Periacinar Clefting and p63 Immunostaining in Prostatic Intraepithelial Neoplasia and Prostatic Carcinoma

Bozo KRUSLIN1, Davor TOMAS2, Aida CVIKO1, Hrvoje CUPIC1, Ljubica ODAK1, Mladen BELICZA1

1Ljudevit Jurak Department of Pathology, Sestre milosrdnice University Hospital, Zagreb, Croatia
2Department of Pathology, Bringham and Women's Hospital, Harvard Medical School, Boston, USA


The aim of the present study was to correlate the presence and extent of retraction clefting and the expression of p63 in neoplastic glands and glands with prostatic intraepithelial neoplasia (PIN) in needle core biopsies. We analyzed needle core biopsies from 28 patients with PIN and 41 patients with adenocarcinoma. Neoplastic glands and those with PIN were analyzed on high power field (400x) and classified in three groups according to the extent of clefting. Immunohistochemical staining was performed following Microwave Streptavidin ImmunoPeroxidase (MSIP) protocol on DAKO TechMate Horizon automated immunostainer. Periacinar retraction clefting was significantly more prominent in prostatic carcinoma compared to PIN (p<0.0001) and nonneoplastic glands (p<0.0001). There was no difference between normal glands and PIN regarding clefting (p=0.8064). p63 was positive around the whole circumference in 12 out of 28 cases with PIN, and discontinuously positive in remaining 16 PIN cases suggesting initial disruption of the basal cell layer. p63 immunostaining was also positive in all nonneoplastic glands, and negative in all carcinomas. We conclude that retraction clefting was associated with cancer and lack of basal cells, but not with PIN. The relationship between clefting and p63 immunostaining in prostatic cancer should be further analyzed. Pathology & Oncology Research, Vol 12, Nr 4, 205-209, 2006

Key words: p63; periacinar clefting; prostatic adenocarcinoma; prostatic intraepithelial neoplasia (PIN)

Received: Mar 20, 2006; accepted: Oct 21, 2006
Correspondence: Bozo KRUSLIN, Ljudevit Jurak Department of Pathology, Sestre milosrdnice University Hospital, Vinogradska 29. Zagreb 10000, Croatia; Tel: 3851 3787 177, Fax: 3851 3787 244; E-mail:

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