Clinical Findings and HER-2/neu Gene Amplification Status of Breast Carcinoma Patients

Feride Iffet SAHIN1, Zerrin YILMAZ1, Mahmut Can YAGMURDUR2, Fatma Belgin ATAC1, Binnaz Handan OZDEMIR3, Hamdi KARAKAYALI2, Beyhan DEMIRHAN3, Mehmet HABERAL2

1Kubilay Sokak No: 36 Maltepe, Department of Medical Biology and Genetics, Baskent University Faculty of Medicine, Ankara, Turkey
2Department of Surgery, Baskent University Faculty of Medicine, Ankara, Turkey
3Department of Pathology, Baskent University Faculty of Medicine, Ankara, Turkey


The study group was derived from the archival materials of 48 invasive intraductal breast cancer patients who had undergone partial mastectomy/ axillary dissection. All patients included in the study had clinically T1-2N0M0 invasive ductal carcinoma. To detect HER-2/neu status, fluorescent in situ hybridization was performed using a HER-2/neu locus-specific probe. Signals were counted and patients were classified in three groups according to signal ratios: signal ratio <2, group 1 (n=31); signal ratio 2-4, group 2 (n=11); signal ratio >4, group 3 (n=6). Ratios of axillary metastatic lymph nodes to dissected total lymph nodes were 17%, 23% and 83% in groups 1, 2 and 3 respectively (P=0.003). The number of metastatic axillary lymph nodes, and the ratio of microscopic metastatic lymph nodes were highest in group 3 (P=0.001 and P=0.008, respectively). No significant difference was observed between groups for distant metastasis in a 5-year follow-up period. Signal ratios decreased with estrogen receptor expression (P=0.03). Histopathologically, an irregular growth pattern of the tumor was observed in 100% of the patients in group 3, and in 54% and 60% in groups 1 and 2, respectively (P=0.04). Lymphovascular invasion of the tumor was significantly higher in group 3 compared to the other two groups (P=0.01). The extensive intraductal component ratio was the highest in group 3 (P=0.04). The appearance of desmoplastic reaction and lymphocyte infiltration did not show significant difference between the groups. Our results show that HER-2/neu signal ratio increases with lymphovascular invasion, an extensive intraductal component, irregular growth pattern and axillary metastasis in clinically T1-2N0M0 invasive ductal carcinoma of the breast. Pathology & Oncology Research, Vol 12, Nr 4, 211-215, 2006

Key words: HER-2/neu; breast carcinoma; FISH

Received: Jan 24, 2006; accepted: Sep 15, 2006
Correspondence: Feride Iffet SAHIN, Kubilay Sokak No: 36 Maltepe, Department of Medical Biology and Genetics, Baskent University Faculty of Medicine, Ankara 06570, Turkey; Tel: +90 312 2324400/138, Fax: +90 312 2319134; E-mail:

Click here to get the full-text version in PDF!