Detection of Bladder Cancer from the Urine using Fluorescence in situ Hybridization Technique

Péter RIESZ1, Gábor LOTZ2, Csilla PÁSKA2, Attila SZENDRŐI1, Attila MAJOROS1, Zsuzsanna NÉMETH2, Péter TÖRZSÖK2, Tibor SZARVAS3, Ilona KOVALSZKY3, Zsuzsa SCHAFF2, Imre ROMICS1, András KISS2

1Department of Urology, Semmelweis University, Budapest, Hungary
22nd Department of Pathology, Semmelweis University, Budapest, Hungary
31st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary


The authors report on their first experiences with the UroVysion fluorescence in situ hybridization (FISH) kit developed for the detection of bladder cancer. This new non-invasive diagnostic application of the FISH technique in the field of urology was elaborated to replace cystoscopy. The special urine examination method detects genetic alterations of the urothelial cells found in the urine, using fluorescent directlabeled DNA probes binding to the peri-centromeric regions of chromosomes 3, 7 and 17 as well as on the 9p21 locus. We aimed to evaluate the utility of UroVysion test in the light of the histological diagnosis. Urine samples from 43 bladder cancer patients and 12 patients with no or benign alterations were studied using a new application of FISH technique: the UroVysion reagent kit. The obtained FISH results were compared with the histological findings of the transurethral surgical resection specimens. The study rated the specificity and sensitivity of the technique 100% and 87%, respectively. Therefore, the technique could well fit into the diagnostic process of bladder carcinomas. Statistical analyses showed significant correlation between tumor progression and the severity of the genetic alterations detected by this FISH technique. Furthermore, positive correlation was found between tumor grade and the proportion of tumor cells showing genetic abnormality. The noninvasiveness, the robustness of evaluation and the high specificity/sensitivity are all in favor of this technique. The disadvantages are the higher costs of the technical background and the required future clinical studies to determine whether this technique can replace cystoscopy. Pathology & Oncology Research, Vol 13, Nr 3, 187-194, 2007

Key words: bladder cancer; cystoscopy; fluorescence in situ hybridization; molecular pathology

Received: Feb 5, 2007; accepted: Sep 5, 2007
Correspondence: Gábor LOTZ, 2nd Department of Pathology, Semmelweis University, Üllõi út 93. Budapest H-1091, Hungary; Tel: 36-1-2156921; E-mail:

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