PATHOLOGY & ONCOLOGY RESEARCHVol. 6 No. 3, 2000

 Article

Analysis of the Expressed Immunoglobulin Variable Region Heavy Chain Gene Products in Paraproteins from Iranian Patients with Multiple Myeloma

Soheila GHARAGOZLOO1, Ramazan A SHARIFIAN2, Rizgar A MAGEED2, Fazel SHOKRI1

1Department of Immunology, School of Public Health Tehran University of Medical Sciences, Tehran, Iran
2Clinic of Hematology and Oncology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

 

The frequency of expression of immunoglobulin (Ig) variable region heavy (VH ) chain gene products was studied in 43 Iranian patients with mutiple myeloma (MM). The expressed VH gene families and associated cross-reactive idiotypes (CRI) were analysed by immunoblotting and ELISA, using peptide-induced polyclonal antibodies specific for VH 1-VH 6 gene families and monoclonal antibodies (MAb) recognising CRI linked to theVH 1, VH 3, VH 4 and VH 6 gene families. The results revealed that the VH 3 family (60.5%) was the most predominant gene family. In contrast, no paraproteins were encoded by genes from the VH 2 gene family and only 2.3% were encoded by the VH 5 family. The panel of paraproteins tested rarely expressed the probed VH -associated CRI. Our results suggest that: 1-The Ig VH genes, may not be randomly expressed in the malignant plasma cells from Iranian patients with MM. 2- Some of the genes seem to be negatively selected or highly mutated, as evidenced by the lack of expression of the probed CRI. Pathology & Oncology Research, Vol 6, Nr 3, 185-190, 2000

Key words: multiple myeloma; VH genes; paraprotein; cross-reactive idiotype


Received: Dec 29, 1999; accepted: Jun 5, 2000
Correspondence: Fazel SHOKRI, Department of Immunology, School of Public Health Tehran University of Medical Sciences, Tehran 14155, Iran, Fax: 021 6462267

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