PATHOLOGY & ONCOLOGY RESEARCHVol. 6 No. 3, 2000

 Article

Tenascin Expression in Primary and Recurrent Breast Carcinomas and the Effect of Tenascin on Breast Tumor Cell Cultures

Anna-Mária TÕKÉS1, Sándor PAKU2, Sára TÓTH3, Edina PAÁL1, Janina KULKA1, József TÓTH4, András TELEKES4

12 nd Department of Pathology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
21 st Department of Pathology and Experimental Cancer Research, Faculty of Medicine, Semmelweis University, Budapest, Hungary
3Department of Genetics, Cell and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
4National Institute of Oncology, Budapest, Hungary

 

Tenascin is generally classified as an anti-adhesive protein. Many cells do not adhere to tenascin or if they adhere they do not spread. In this study we analysed the stromal expression of tenascin-C in primary, second primary and recurrent breast carcinomas and the ability of tenascin-C to stimulate the focal adhesion plaques in MDA-MB-435 breast carcinoma cell line. To assess the tenascin-C expression formalin-fixed, paraffin-embedded specimens of 20 specially selected breast carcinomas and their recurrences (14) or a second primary breast cancer of the same patient (6) were examined with immunohistochemical methods. We also studied the effect of tenascin-C on focal adhesion plaques added to MDA-MB-435 breast carcinoma cell line. During a median 2,9-year patient follow up 14 local recurrences and 6-second primary breast carcinomas developed in the 20 patients. In 3 cases a second recurrence occurred. The presence of tenascin in tumor cells, in the proliferating and some normal ducts, near to the tumor cell nests, in the stroma and in ductal carcinoma in situ component of the invasive carcinoma may suggest the role of tenascin played in tumor cell migration. Soluble tenascin added to the cell culture had minimal or no effect on focal adhesion plaques. Tenascin only seems not to be of prognostic value in predicting the local recurrence of breast cancer. Pathology & Oncology Research, Vol 6, Nr 3, 202-209, 2000

Key words: tenascin; breast cancer; cell cultures; focal adhesion plaques


Received: Jan 25, 2000; accepted: Jun 5, 2000
Correspondence: Anna-Mária TÕKÉS, 2 nd Department of Pathology, Faculty of Medicine, Semmelweis University, Üllôi út 93. Budapest H-1091, Hungary; Tel: 36-1-215-7300, Fax: 36-1-215-6921/3402

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